How do you calculate isothermal titration calorimetry?
Making a measurement The injection device is inserted into the sample cell containing the protein of interest. A series of small aliquots of ligand are injected into the protein solution. If there is a binding of the ligand to the protein, heat changes of a few millionths of a degree Celsius are detected and measured.
What is PEAQ ITC?
The MicroCal PEAQ-ITC is a highly sensitive, low volume isothermal titration calorimeter for the label-free in solution study of biomolecular interactions. It delivers direct measurement of all binding parameters in a single experiment and can analyze weak to high affinity binders, using as little as 10µg sample.
How much protein is typically required for an ITC experiment?
No immobilization required → measurements in solution. Low sample consumption for ITC standards → only 300 µl of a 20 µM protein solution required.
How sensitive is ITC?
What is ITC experiment?
Isothermal titration calorimetry (ITC) is a well established technique that can determine all the thermodynamic parameters (affinity, enthalpy and stoichiometry) of a binding interaction in one experiment. The signal measured is the heat released or absorbed upon interaction (binding) of the two reactants.
What affects binding affinity?
Binding affinity is influenced by non-covalent intermolecular interactions such as hydrogen bonding, electrostatic interactions, hydrophobic and Van der Waals forces between the two molecules. In addition, binding affinity between a ligand and its target molecule may be affected by the presence of other molecules.
Is bomb calorimeter isothermal?
The system was a full isothermal system with water jacket. Prior to development of the CP500, it was demonstrated by Digital Data Systems that a polystyrene jacket, which has very little thermal mass and negligible heat conductivity, could replace the water jacket. The system was referred to as the “dry method”.
Who invented bomb calorimeter?
Pierre Eugene Berthelot
The next milestone takes us to Pierre Eugene Berthelot, French chemist, who built the first modern bomb calorimeter in the 1870-s. He is also credited with inventing the concept of endothermic and exothermic reactions [6].
What is isothermal titration calorimetry (ITC)?
Isothermal Titration Calorimetry (ITC) is a label-free method for measuring binding of any two molecules that release or absorb heat upon binding. ITC can be used to measure the thermodynamic parameters of biomolecular interactions, including affinity (KA), enthalpy (ΔH), entropy (ΔS), and stoichiometry (n).
What is the range of MicroCal microcalorimeters?
The range includes both Differential Scanning and Isothermal Titration calorimetry. MicroCal microcalorimeters are used in a wide range of applications from basic research to the discovery and development of small molecule drugs, biotherapeutics and vaccines.
What can micromicrocal ITC ITC be used for?
MicroCal ITC isothermal titration calorimeters are used to study a wide range of biomolecular interactions. The applications range from drug design to fundamental research such as the understanding and regulation of signal transduction pathways.
How does the itc200 microcalorimeter work?
The ITC200 microcalorimeter has two cells: one contains water and acts as a reference cell, the other contains the sample. The microcalorimeter needs to keep these two cells at exactly the same temperature during the course of an experiment.