Which equipment are necessary for animal cell culture laboratory?
What are the Basic equipments required for animal cell culture?
| Essential equipments | Beneficial equipments | Useful additional equipments |
|---|---|---|
| Incubator | Laminar flow hood | Low-temperature freezer |
| Microscope | Cell counter | Glassware washing machine |
| Sterilizer | Vacuum Pump | Colony counter |
| Washing up instrument | CO2 incubator | Closed-circuit machine |
What equipment is used to culture cells?
One of the essential pieces of equipment for any lab is a heat resistant Erlenmeyer flask. These flasks will be used for preparing the tissue culture media. Other equipment pieces include a small capacity flask and a culture jar.
Which equipment is used for sterilization of instruments of animal tissue culture lab?
The laboratory apparatus designed to use steam under regulated pressure is called an autoclave. The autoclave is an essential unit of equipment in every microbiology or cell culture laboratory. Many media, solutions, discarded cultures, and contaminated materials are routinely sterilized with this apparatus.
What are the techniques used in animal cell culture?
Biologicals produced by recombinant DNA (rDNA) technology in animal cell cultures include anticancer agents, enzymes, immunobiologicals [interleukins, lymphokines, monoclonal antibodies (mABs)], and hormones. Animal cell culture has found use in diverse areas, from basic to advanced research.
What materials and equipment are used in tissue culture?
Basic Equipment
- Cell culture hood (i.e., laminar-flow hood or biosafety cabinet)
- Incubator (humid CO2 incubator recommended)
- Water bath.
- Centrifuge.
- Refrigerator and freezer (–20°C)
- Cell counter (e.g., Countess® II FL Automated Cell Counter or hemacytometer)
- Inverted microscope.
What are the basic precautions needed for cell culture work?
– Clean laboratory equipment routinely, even if it is not contaminated. – Avoid the creation of aerosols and/or splashes. – Wash your hands after working with potentially hazardous materials and before leaving the laboratory. – Decontaminate all potentially infectious materials before disposal.
What 2 equipment can we use to see cells in the lab?
The two most common methods for visualizing living cells are phase-contrast microscopy and differential interference-contrast microscopy (Figure 1.26).
Which technique is used for sterilization of animal cell culture media?
The role of filtration Filtration is commonly used to protect cell culture media from contamination.
Which method is used mostly for histology of animal tissue?
Microscopic anatomy techniques such as staining are used for determining the histology and histochemistry of animal tissue.
Which machine is used for inoculation to prohibiting unwanted growth in PTC?
To prevent growth of the micro-organisms the medium can be sterilised in an autoclave at 15 lb pressure at 120°C for 15 minutes.
What are the applications of animal tissue culture?
The nine applications of animal cell culture are: (1) Model Systems (2) Toxicity Testing (3) Cancer Research (4) Virology (5) Cell-Based Manufacturing (6) Genetic Counselling (7) Genetic Engineering (8) Gene Therapy and (9) Drug Screening and Development.