What is the structure of peroxidase?
Peroxidase are enzymes that catalyze oxidation-reduction reaction by mechanism of free radical that transform several compounds into oxidized or polymerized products. The prosthetic group of peroxidase is composed of a protein-bound heme, usually through a histidine residue that acts as a proximal ligand.
What are the products of peroxidase?
Hydrogen peroxidase is an enzyme found in all aerobic cells, which functions to decompose toxic hydrogen peroxide (Petersen and Anderson, 2005). The products of this reaction are water and oxygen gas. Enzymes are complexes produced by living cells, which catalyze chemical reactions in organic matter.
What is the role of peroxidase in the ELISA test?
One commonly used enzyme conjugate in ELISA is horseradish peroxidase. Horseradish peroxidase (HRP) is a 40,000 Dalton protein, which catalyzes the reduction of hydrogen peroxide (H2O2) to water (H2O) [1]. Amplex® Red is a substrate for use with HRP containing assays [2].
What is horseradish peroxidase HRP and what does it do?
Horseradish peroxidase (HRP) is an enzyme used to amplify signal in photometric assays by catalyzing the conversion of chromogenic or chemiluminescent substrates for the detection of targets such as proteins, carbohydrates, and nucleic acids.
Is peroxidase an oxidase?
The remarkable property of peroxidases to act as aerobic oxidases in the presence of dihydroxymaleic acid has led to the view that peroxidase acts as an oxidase by means of a ferric-ferrous cycle of valency change as do the cytochromcs (1).
What is the meaning of peroxidase?
Definition of peroxidase : an enzyme that catalyzes the oxidation of various substances by peroxides.
What is peroxidase test?
Thyroid peroxidase test is a test that measures the level of an antibody that is directed against thyroid peroxidase (TPO). Autoantibodies to thyroid peroxidase (TPOAb) are produced within the body. The presence of TPOAb in the blood reflects a prior attack on the thyroid tissue by the body’s immune system.
How did peroxidase get its name?
Peroxidases or peroxide reductases (EC number 1.11. 1. x) are a large group of enzymes which play a role in various biological processes. They are named after the fact that they commonly break up peroxides.