What is the difference between Nucleofection and electroporation?

What is the difference between Nucleofection and electroporation?

Based on the physical method of electroporation, nucleofection uses a combination of electrical parameters, generated by a device called Nucleofector, with cell-type specific reagents. In contrast, other commonly used non-viral transfection methods rely on cell division for the transfer of DNA into the nucleus.

What is Amaxa?

The Amaxa Nucleofector technology is a transfection system designed to transfer genes into cells quickly and efficiently. The technology is based on electroporation. Each cell type is introduced to the DNA (or RNA) in an optimized Nucleofector solution that has been designed for that particular cell type.

What is stable transfection?

Stable transfection refers either to the permanent expression of the gene of interest through the integration of the transfected DNA into the nuclear genome, or the maintenance of a transfected plasmid as an extra chromosomal replicating episome within the cell.

What is the efficiency of lipofectamine transfection?

Lipofectamine and nucleofection of HEK 293 were essentially equivalent in terms of toxicity and efficiency. Transient transfection efficiency in all the cell systems ranged from 40%-90%, with minimal toxicity and no apparent species specificity.

Is nucleofection more effective than chemical transfection reagents?

The results presented here indicate that nucleofection is more effective than chemical transfection reagents from several different cationic categories (dendrimer, polyethylenimine, lipid) at delivering DNA into a variety of different cell types.

How effective is electroporation in transfection?

Electroporation via the nucleofector machine was the most effective method tested. It exhibited a 10 to 20 fold (for SMC and EC, respectively) increase in transfection efficiency in comparison to the lipofection method combined with acceptable toxicity.

What is transfection and how does it work?

Transfection is a process by which foreign nucleic acids are delivered into a eukaryotic cell to modify the host cell’s genetic makeup ( Kim & Eberwine, 2010; Chow et al., 2016 ).