What is MES buffer used for?
MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography.
What is MES free acid?
It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH.
Is MES an acid?
MES is the common name for the compound 2-(N-morpholino)ethanesulfonic acid. Its chemical structure contains a morpholine ring….MES (buffer)
| Names | |
|---|---|
| Chemical formula | C6H13NO4S |
| Molar mass | 195.2 g/mol |
| Acidity (pKa) | 6.15 |
| Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). |
What is 2 -( N morpholino ethanesulfonic acid?
Description. 2-(N-morpholino)ethanesulfonic acid is a Good’s buffer substance, pKa = 6.15 at 20 ℃. It is an organosulfonic acid and a MES. It is a conjugate acid of a 2-(N-morpholino)ethanesulfonate. It is a tautomer of a 2-(N-morpholiniumyl)ethanesulfonate.
Can you autoclave MES?
Autoclaving is not recommended for any sulfonic acid buffer. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). When MES solutions are stored at room temperature, they can turn yellow within six months.
How do you get pH MES?
MES (0.5 M, pH 6) Preparation and Recipe
- Prepare 800 mL of dH2O in a suitable container.
- Add 97.62 g of MES free acid to the solution.
- Starting pH for 0.5M MES solution is 3.23 For 1 L of 0.5 M MES, 13.6 ml of 10N NaOH is needed to adjust the pH to 6.0.
- Add dH2O until volume is 1 L.
How does the MES system work?
A MES helps guide production operators step by step, through all the tasks required to transform raw materials into finished goods. It does this using digital work instructions, production routings and connecting to production machinery to build a traceable record of the manufacture of products within a factory.
What is the difference between MES and MES hydrate?
Note: MES Hydrate (CAS# 1266615-59-1) and MES Monohydrate (CAS# 145224-94-8) differ only in the number of water molecules associated with the buffer in powder form. Regardless of the number of water molecules present, these buffers are identical when dissolved into an aqueous solution.
What is MES salt?
MES, Sodium Salt (2-(N-Morpholino)ethanesulfonic acid sodium salt) is a zwitterionic N-substituted aminosulfonic acid known as a Good’s buffer, active in the pH range 6-8 with a pKa of 6.10 at 25° C. Also used in cation-exchange chromatography and as a biological buffer in plant cell cultures.
How do I store MES buffer?
I agree with Bruce Allen that it would be wise to discard the buffer and make or buy a fresh batch. Store it at 4oC protected from light and it should last a long time without turning yellow. Cover the bottle with aluminum foil to prevent degradation. I use dark colored bottle for all light sensitive reagents.