What is Czapek Dox agar used for?
Czapek Dox Agar is a semisynthetic medium containing sodium nitrate as the sole source of nitrogen. It is used for the cultivation of fungi. This medium can also be used for chlamydospore production by Candida albicans. The medium has good buffering action due to the presence of different salts.
How do you make Czapek Dox Agar?
Preparation and Method of Use of Czapek’s Agar (CZA)
- Suspend 49.01 grams in 1000 ml distilled water.
- Heat to boiling to dissolve the medium completely.
- Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
- Cool to 45-50°C.
- Mix well and pour into sterile Petri plates.
What is CDA medium?
Czapek medium, also called Czapek’s agar (CZA) or Czapek-Dox medium, is a growth medium for propagating fungi and other organisms in a laboratory. It works well for many saprophytic fungi and soil bacteria such as species of Aspergillus, Candida, Penicillium, and Paecilomyces.
How do you make Czapek Dox broth?
Czapek Dox Broth is a semisynthetic medium used for the general cultivation of fungi. Suspend 35.01 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
What is Czapek Dox broth?
Czapek Dox Broth is a semi-synthetic medium used for the cultivation of fungi, containing sodium nitrate as the sole source of nitrogen. This medium is prepared according to the formula developed by Thom and Church (1), which has a defined chemical composition.
What is in Czapek Dox?
Czapek Dox Agar (Modified) is a medium containing sodium nitrate as the sole source of nitrogen, it is one of the most useful solid media for the general cultivation of fungi. In the Oxoid medium magnesium glycerophosphate and potassium sulphate replace the magnesium sulphate and potassium phosphate of the original.
How do you make agar water?
Suspend 20.00 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Dispense as desired.
What is malt extract agar used for?
Malt Extract Agar is used for the cultivation of fungi and is not intended for use in the diagnosis of disease or other conditions in humans. An acidic medium which will support the growth of most yeasts and molds whilst inhibiting most bacteria.
What is water agar medium?
Water Agar is used for enumeration of fungi according to MPN method. Successful isolation of the fungi can be achieved by the use of selective media that slow down the growth of the fungi. Most fungi and bacteria will grow on Water Agar, but at such a slow rate that it is relatively easy to isolate the target fungus.
Is water agar medium selective?
Water Plate Count Agar is a non-selective medium which conforms to ISO 6222:1999, and is not intended for use in the diagnosis of disease or other conditions in humans. Water Plate Count Agar allows the enumeration of culturable microorganisms, which can be used for the assessment and surveillance of water quality.
Can bacteria grow on malt agar?
Malt extract agar is a light creamy colored medium. It has an acidic pH (pH 4.7 ± 0.2) which allows for optimal growth of yeasts and molds, while restricting bacterial growth. While Malt Extract supports the growth of yeasts and molds and inhibits bacteria, Nutrient-TTC encourages bacterial growth.
How long does malt extract agar last?
Can be stored in the fridge for up to 90 days before using.