What does anti-A1 lectin positive mean?
Agglutination
Agglutination of the red blood cells is a positive test result and indicates the presence of the A1 antigen. No agglutination of the red blood cells is a negative test result and indicates the absence of the A1 antigen.
What lectin has anti-A1 specificity?
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| Lectin | Blood Group Specificity | Quantity |
|---|---|---|
| Salvia horminum (SHA) | Anti-T + Cad | 2ml |
| Salvia sclarea (SSA) | Anti-Tn | 2ml |
| Sophora japonica (SJA) | Anti-B | 2ml |
| Ulex europaeus (UEA-I) | Anti-O(H) | 2ml |
What is the source of anti-A1?
This anti-A1 reagent is an extract from the seeds of Dolichos biflorus and can be used to differentiate A1 human red cells from other subgroups of A when tested by the tube technique.
What does dolichos Biflorus react with?
Dolichos biflorus agglutinin (DBA) binds to N-acetyl-D-galactosamine (GalNAc) residues in glycoconjugates and agglutinates erythrocytes carrying blood group antigen A.
What is the difference between a positive and A1 positive?
The blood group A1 is the sub type of A and it may have positive or negative in Rh. Blood group A is in two of the major blood groups: O, A, B and AB. Group A is made up of subtypes of A, i.e. A1, A2, and rare subtypes such as A3, Aint, etc. So, subtype A1 is in (and is similar to) blood group A.
Is A1 positive rare?
A1 and A2 are rare subgroups. However, they may lead to transfusion related reactions which could be lethal. By knowing the prevalence of A1 and A2 subgroups in a blood bank at tertiary care hospital, the dangerous transfusion reactions occurring due to these minor incompatibilities can be avoided.
What is anti-A1 antibody?
• Anti-A1 alloantibodies are usually clinically insignificant naturally occurring cold IgM antibodies that can occur in some A-subgroup patients. • Anti-A1 have been reported to be clinically significant if the antibody is reactive at 37 °C or body temperature on immunohematology testing.
Is anti-A1 clinically significant?
In most cases, anti-A1 is of no clinical significance, reacting well below body temperature, and is merely a laboratory nuisance causing ABO discrepancies. When anti-A1 is active at body temperature, though rare, extensive destruction of A1 cells in vivo can occur and has been documented.
What are anti-A1 antibodies?
What is lectin dolichos Biflorus?
General description. Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.
What does A1 blood type mean?
Subtypes for blood type A include A1, A2, Ax, Aint and others, but the most common is A1. • If the donor is not subtype A1, it means they have less A antigen on their red blood cells (RBCs) and organs, which allows them to donate to recipients outside of their primary blood type.
What is Anti A1 lectin used for?
ORTHO Anti-A1 Lectin (Dolichos biflorus) is designed for use in agglutination tests for the recognition of the A1. antigen on human red blood cells. This reagent is a purified extract of the seeds of Dolichos biflorus, containing. a phytohemagglutinin which agglutinates red blood cells of the subgroup A1 or A1B.
What is Anti H lectin used for?
The Anti-H Lectin is a purified extract from seeds of Ulex europaeus. Anti-H Lectin is used to demonstrate the presence of H antigen on human red blood cells and in assessing the H secretor status of group ‘O’ individuals. 20 Related Question Answers Found Is A and a1 blood group the same?
Why are lectins used as an anti-B reagent?
The application of lectins as an anti-B reagent has proven to be as useful as human polyclonal or mouse monoclonal antibodies. Besides their specificity, lectins are excellent reagents because of their lower cost and indigenous production.
Does anti-a1 serum agglutinate A2 cells?
Serum containing anti-A1, whether it is naturally occurring or passively transfused, will agglutinate all A1 cells, but not A2 cells or any other subgroup of A. The serum of group A1 patients does not contain anti-A1 unless it has been passively transfused from a non-group A blood component, such as group O platelets.