How much glucose is high in DMEM?
This product – DMEM High Glucose – has an increased glucose content of 4.5 g/L and is supplemented with L-glutamine. Dulbecco’s Modified Eagle Medium is based on Eagle’s Minimal Essential Medium (EMEM) but has a fourfold higher concentration of amino acids and vitamins.
Why is DMEM high in glucose?
The DMEM original formulation contains 1000 mg/l of glucose, and is used to culture embryonic mouse cells. The use of 4500mg/l of glucose in DMEM High Glucose shows an optimal cell growth for some cell lines.
Does DMEM contain insulin?
Higher doses of insulin (20.0 micrograms/ml) in low-serum DMEM were able to stimulate growth of these cells compared with controls. In low-serum DMEM containing 2.5 micrograms/ml insulin there was a dose-dependent increase in cell growth with EGF and TFG alpha at concentrations of 0.5-10.0 nM.
Does DMEM contain glucose?
DMEM/Ham’s Nutrient Mixture F-12 (50:50) contains 17.5 mM of D-glucose; DMEM (Hi), GMEM and IMDM all contain 25 mM levels of D-glucose; and H-Y Medium (Hybri-Max®) and Serum-Free/Protein Free Hybridoma Medium contain 22.6 and 28.9 mM D-glucose, respectively.
What is DMEM used for in cell culture?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
What does DMEM stand for?
DMEM (Dulbecco’s Modified Eagle Medium) is the most broadly suitable medium for many adherent cell phenotypes among defined media for cell and tissue culture.
What is low glucose DMEM?
DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
How much does DMEM cost?
Refine
| Product | Product Name | Price |
|---|---|---|
| SH30022.LS | DMEM/High glucose with L-glutamine; without sodium pyruvate – 6 x 1000 mL | 192.19 USD |
| SH30022.01 | DMEM/High glucose with L-glutamine; without sodium pyruvate – 500 mL | 22.25 USD |
| SH30022.FS | DMEM/High glucose with L-glutamine; without sodium pyruvate – 6 x 500 mL | 120.07 USD |
What is DMEM made of?
DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.
What is the cost of GIBCO DMEM?
Gibco™ DMEM, high glucose, no glutamine, no phenol red Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, No Glutamine, No Phenol Red is used in cell culture processing applications. $30.77 – $266.00
What is the difference between Eagle’s DMEM and DMEM?
Using DMEM. DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Does DMEM need to be mixed with FBS?
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
How much CO2 does dmdmem use?
DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician.