How much can a 384-well plate hold?
The Standard Thermo Scientific 384-well plate is compatible with the majority of 384 block PCR and sequencing instruments. It has a maximum well volume of 40 µL and a working volume of 25 µL.
How many cells are in a 384-well plate?
13. 500 cells / µL (15,000 cells/well/30 µL), or 500,000 cells/mL, 7 mL of cells is sufficient for half of a 384- well plate, so roughly 3.5 million cells are required to plate at a density of 15,000 cells/well in half of a 384-well plate.
How many UL is a 384-well plate?
HardShell PCR Plates
| Well format | 96-well | 384-well |
|---|---|---|
| Well volume | 200 µL | 50 µL |
| Recommended working volume | 150 µL-160 µL | 37.5 µL-40 µL |
| Height (mm) | 16.06 | 10.40 |
| Length (mm) | 127.76 | 127.76 |
What are the dimensions of a 384 well plate?
384 flat well microtiter plate, without lid, rounded square well design, alphanumeric well coding; length: 127.76 +/- 0.15 mm; width: 85.48 +/- 0.15 mm; curvature: approx. 150 μm.
How much volume does a 24 plate hold?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Growth medium (mL). Approx. volume | |
|---|---|---|
| Dishes | ||
| 24-well | 142475 | 0.5 to 1.0 |
| 48-well | 150687 | 0.2 to 0.4 |
| 96-well | 167008 | 0.1 to 0.2 |
How much volume does a 24 well plate have?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Trypsin (mL of 0.05% trypsin, 0.53 mM EDTA). Approx. volume | |
|---|---|---|
| Dishes | ||
| Flasks | ||
| T-25 | 156367;156340 | 3 |
| T-75 | 156499;156472 | 5 |
How much volume does a 6-well plate have?
i.e. A well in a 6-well dishe has a surface area of 9.6cm2. The minimum volume should be 1.92ml and the maximum 4.8ml.
Are all 96-well plates the same size?
The length of the 96-well standard microplate is 127.71 mm x the width 85.43 mm and the height is 14.10 mm (shown in fig….ANSI- /SLAS – Standard 1 to 4 – 2004.
| 96-Well Standard Plate Dimensions | |
|---|---|
| W | 85.43 mm |
| H | 14.10 mm |
| X1 | 14.36 mm |
| Xn | 113.36 mm |
How do you find the volume of a plate?
Calculate or measure the volume of the plate; if the plate density is known, divide the plate weight by the density. For example, the plate is made of aluminum (the density 2.7 g/cubic cm) and weighs 41.85 g. Then the plate volume is 41.85 / 2.7 = 15.5 cubic cm.
How much trypsin is in a 96-well plate?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Trypsin (mL of 0.05% trypsin, 0.53 mM EDTA). Approx. volume | |
|---|---|---|
| Dishes | ||
| 96-well | 167008 | 0.05 to 0.1 |
| Flasks | ||
| T-25 | 156367;156340 | 3 |