How much can a 384-well plate hold?

How much can a 384-well plate hold?

The Standard Thermo Scientific 384-well plate is compatible with the majority of 384 block PCR and sequencing instruments. It has a maximum well volume of 40 µL and a working volume of 25 µL.

How many cells are in a 384-well plate?

13. 500 cells / µL (15,000 cells/well/30 µL), or 500,000 cells/mL, 7 mL of cells is sufficient for half of a 384- well plate, so roughly 3.5 million cells are required to plate at a density of 15,000 cells/well in half of a 384-well plate.

How many UL is a 384-well plate?

HardShell PCR Plates

Well format 96-well 384-well
Well volume 200 µL 50 µL
Recommended working volume 150 µL-160 µL 37.5 µL-40 µL
Height (mm) 16.06 10.40
Length (mm) 127.76 127.76

What are the dimensions of a 384 well plate?

384 flat well microtiter plate, without lid, rounded square well design, alphanumeric well coding; length: 127.76 +/- 0.15 mm; width: 85.48 +/- 0.15 mm; curvature: approx. 150 μm.

How much volume does a 24 plate hold?

Useful information for various sizes of cell culture dishes and flasks

Catalog No. Growth medium (mL). Approx. volume
Dishes
24-well 142475 0.5 to 1.0
48-well 150687 0.2 to 0.4
96-well 167008 0.1 to 0.2

How much volume does a 24 well plate have?

Useful information for various sizes of cell culture dishes and flasks

Catalog No. Trypsin (mL of 0.05% trypsin, 0.53 mM EDTA). Approx. volume
Dishes
Flasks
T-25 156367;156340 3
T-75 156499;156472 5

How much volume does a 6-well plate have?

i.e. A well in a 6-well dishe has a surface area of 9.6cm2. The minimum volume should be 1.92ml and the maximum 4.8ml.

Are all 96-well plates the same size?

The length of the 96-well standard microplate is 127.71 mm x the width 85.43 mm and the height is 14.10 mm (shown in fig….ANSI- /SLAS – Standard 1 to 4 – 2004.

96-Well Standard Plate Dimensions
W 85.43 mm
H 14.10 mm
X1 14.36 mm
Xn 113.36 mm

How do you find the volume of a plate?

Calculate or measure the volume of the plate; if the plate density is known, divide the plate weight by the density. For example, the plate is made of aluminum (the density 2.7 g/cubic cm) and weighs 41.85 g. Then the plate volume is 41.85 / 2.7 = 15.5 cubic cm.

How much trypsin is in a 96-well plate?

Useful information for various sizes of cell culture dishes and flasks

Catalog No. Trypsin (mL of 0.05% trypsin, 0.53 mM EDTA). Approx. volume
Dishes
96-well 167008 0.05 to 0.1
Flasks
T-25 156367;156340 3