How is a SIM test performed?

How is a SIM test performed?

Procedure of SIM Test Take pure colonies from an 18-24-hour old culture on a solid medium. Inoculate the SIM Medium by stabbing the center of the medium to a depth of half an inch. Incubate the inoculated medium aerobically at 37°C for 18-24 hours. Observe for hydrogen sulfide production and motility of test organism.

What is SIM biochemical test?

It tests the ability of an organism to do several things: reduce sulfur, produce indole and swim through the agar (be motile). SIM is commonly used to differentiate members of Enterobacteriaceae. Bacteria that have the enzyme tryptophanase, can convert the amino acid, tryptophane to indole.

What is a positive Sim test?

Positive: Darkening of the medium (a black precipitate) or blackening of the line of inoculation indicates the presence of bacteria producing hydrogen sulfide.

How do I read my sim test results?

Interpretation of Results: A blackening of the medium along the line of inoculation indicates a positive H2S test. The absence of any blackening indicates a negative H2S test. A diffuse zone of growth flaring from the line of inoculation indicates a positive motility test.

How many separate tests are there in the SIM test?

Stands for Sulfide, Indole, and Motility. It is a medium used for these three tests. What is the SIM test designed for? To detect three biochemical properties of a bacterium from the inoculation of one SIM tube.

Why is IMViC test done?

The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group. The presence of some coliforms indicate fecal contamination. The term “IMViC” is an acronym for each of these tests.

What is h2s test?

Hydrogen sulfide is produced if the sulfur compound is reduced by the bacterial strain. This test thus determines whether the microbe reduces sulfur-containing compounds to sulfides during the process of metabolism.

How can I check my SIM motility?


  1. Touch a straight needle to a colony of a young (18- to 24-hour) culture growing on agar medium.
  2. Stab once to a depth of only 1/3 to ½ inch in the middle of the tube.
  3. Incubate at 35°-37°C and examine daily for up to 7 days.
  4. Observe for a diffuse zone of growth flaring out from the line of inoculation.

What 3 things does the SIM Agar deep test for?

SIM Deep. SIM tubes test for 3 things: (1) hydrogen sulfide production (2) indole production, and (3) motility.

What is the full form of IMViC?

The term “IMViC” is an acronym for each of these tests. “I” is for indole test; “M” is for methyl red test; “V” is for Voges-Proskauer test, and “C” is for citrate test. The lower case “i” is merely for “in” as the Citrate test requires coliform samples to be placed “in Citrate”.

What is biochemical test IMViC?

Biochemical Test (IMViC) : It is a biochemical series of tests including the following tests: Indole, Methyl Red (MR), Voges-Proskauer (VP), and Citrate. All of the series was designed to differentiate among the Enterics (family Enterobacteriaceae). M = Methyl red test for acid production from glucose.