How are cells prepared for cytospin?
Cytospin preparations A cytospin is obtained by employing centrifugal force to isolate, concentrate and deposit a monolayer of cells from a dilute cell suspension onto a circular area on a slide. The objective is to keep cells intact enabling the morphology of the cells to be examined.
What is cytospin in cytology?
A cytocentrifuge, sometimes referred to as a cytospin, is a specialized centrifuge used to concentrate cells in fluid specimens onto a microscope slide so that they can be stained and examined.
Can you cytospin fixed cells?
Hence, cytospins are often used to immobilize cells onto glass microscope slides. Following this, cells are fixed and can be used for immunofluorescence, immunocytochemistry, or with other stains or dyes.
How do you get cells to stick to a slide?
You can fix your cells in suspension. Next, wash the cells and resuspend them in a low volume of PBS (50-100ul) at room temp. Fill a cryomold with OCT (400-450ul). Get all your cells in a syringe (no air bubbles).
How do you make a cell block?
The cell block is prepared from the pellet of centrifuged cell suspension by adding plasma and thrombin to enmesh the cellular material in a clot (Fig. 1). Add up to 20 mL of specimen to a falcon tube and centrifuge for 10 minutes at 1,650 rpm. After centrifugation, the supernatant is decanted.
What is cytospin and function?
The Cytospin centrifuge is a special purpose instrument designed to deposit cells evenly onto a glass slide. The instrument when used correctly produces a monolayer cell deposition in a defined area of the slide using centrifugal force.
What is the indication of an impression smear?
Impression smears, like skin scrapings, are good for detecting the presence of inflammation, infectious organisms, and abnormal tissue cells.
How do you prepare cytospin slides?
Cytospin preparations A cytospin is obtained by employing centrifugal force to isolate, concentrate and deposit a monolayer of cells from a dilute cell suspension onto a circular area on a slide. The objective is to keep cells intact enabling the morphology of the cells to be examined.
What is the cytospin technique used for?
The cytospin technique can be used on any single cell suspensions of any source such as peripheral blood mononuclear cells (PBMCs), effusions, cerebral spinal fluid (CSF), bronchial lavages, fine-needle aspirates, culture cells, etc.
How much cytospin should be in a suspension?
Remember that the Cytospin must always be balanced. Prepare a cell suspension of not more than 0.5 x 10 6 cells/ml in the protein-containing medium. Note that the optimal concentration of cells may have to be determined for each cell line.
How do you centrifugate cells in cytospin?
Place the mounted holder in the corresponding recess in the Cytospin. A maximum of twelve slides can be centrifuged at once. Remember that the Cytospin must always be balanced. Prepare a cell suspension of not more than 0.5 x 10 6 cells/ml in the protein-containing medium.